2016/2/3 · We combined ultrasound with bactofugation of milk to achieve the microbiological accuracy that is equivalent to pasteurization.Experimental approachThe milk samples (200 mL) were treated for 2.5, 5, 7.5 and 10 min with high-power ultrasound (200 and 400 W) with a frequency of 24 kHz. The treatments were conducted with a constant duty cycle of 100%.