Here we describe an in-vial dual extraction (IVDE) method, with reversed phase and hydrophilic liquid interaction chromatography (HILIC) which reproducibly measured over 4,000 metabolite features
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Here we describe an in-vial dual extraction (IVDE) method, with reversed phase and hydrophilic liquid interaction chromatography (HILIC) which reproducibly measured over 4,000 metabolite features
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Mar 08, 2002 · The residue was transferred to an autosampler vial insert and 18 μl injected onto the HPLC column.2.4. Liquid chromatographyChromatography was performed at ambient temperature with a mobile phase (sparged with helium) consisting of acetonitrile–0.02% aqueous formic acid (300:700, v/v) at a flow-rate of 0.20 ml/min. 2.5. Mass spectrometry
Jan 01, 2009 · The extracts were mixed and dried under a gentle nitrogen stream, then redissolved in 1 mL of methanol. Each final extract was then filtered through a 0.45 μm membrane filter into a 2 mL amber glass vial. The vials were kept at − 18 °C until analysis.The water from Liuxi Reservoir was collected and used for the recovery tests.
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into 10mL amber vials with 9mL saturated NaCl solution. Prior to use, the stir bars were conditioned in the thermal desorption unit (TDU) for 1h at 280 C in a flow of helium. For sample extraction using SBSE, one stir bar was used for 2h at 1000rpm in each screw-capped vial. After extraction, the
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