Jul 20, 2020 · 771 autosampler vial. 772 NanoLC MS/MS Analysis. Peptide mixtures were analyzed by nanoflow liquid 773 chromatography-tandem mass spectrometry (nanoLC-MS/MS) using a nano-LC 774 chromatography system (UltiMate 3000 RSLCnano, Dionex, Thermo Fisher Scientific, 775 San Jose, CA). The nano-LC-MS/MS system was coupled on-line to a Thermo Orbitrap