Each solution was allowed to equilibrate in an HPLC vial (12 x 32 mm) with a teflon-lined snap cap. Samples containing a cylindrical micro-stirrer bar (2 x 5 mm, teflon-coated, manufactured by Cowie obtained from Fisher) were stirred at rpm using an IKA RCT basic hotplate stirrer. Shaken samples were placed in an Eppendorf
HPLC. This vial delivers maximum sample capacity (~1 mL) with minimum residual volume (~9 μL). Waters Maximum Recovery Vial Specifically designed for the bottom draw-port needle of the Waters ACQUITY UPLC and Alliance HT HPLC Systems. This vial delivers maximum sample capacity (~1.5 mL) with minimum residual volume. The 9 mm cap
• ™LightPipe Flow Cell, 2 µL 10 mm (P/N 6083.0100) Thermo Scientific ™ Virtuoso ™ Vial Identification System (P/N 60180-VT-100) Software Thermo Scientific ™ Chromeleon ™ Chromatography Data System 7.2 SR4 Sample preparation Solutions of the nine compounds shown in Table 1 were prepared by dissolving a known amount in mobile
Choose from these Thermo Scientific™ 10mm Clear Glass Wide Opening Screw Thread Vials that are manufactured from clear, (Type 1 Class A) glass and have a 10-425 thread finish.
The analytes were separated on a C12 column using a (95:5% v/v) methanol:10 mM ammonium formate, pH 3.5 mobile phase with a run time of 5 min. Detection was realized through a dual reagent
Typically the wavelength range used in UV detection for HPLC is in the range 200 – 400nm, which covers both UV and the lower part of the visible spectrum. Figure 1 shows the electromagnetic spectrum and the relationship between the wavelength of light and frequency. It should be noted that the shorter the wavelength the higher the energy of
Oct 27, 2021 · Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. x
Apr 08, 2015 · In-vial dual extractions were performed in amber glass HPLC vials with fixed 0.4 mL inserts (Chromacol: Welwyn Garden City, UK). Samples Experimental tissue material was obtained from the cerebellum of adult male (Sprague-Dawley) rats obtained from Harlan Laboratories UK.
The HPLC column used was a Perfect Bond ODSHD C18 analyti- - cal column (100 mm × 4.6 mm i.d., particle size 5 µm), equipped with a guard column (10 mm 4.6× mm, particle size 5 µm) containing the same packing material.
The positive ion TurboIonspray Q1 mass spectrum and product ion mass spectrum of atorvastatin are shown in Fig-ure 1 and Figure 2, respectively. The [M + H]+ was the pre-dominant ion in the Q1 spectrum, and was used as the pre-cursor ion to obtain product ion spectra. The most sensitive mass transition was from m/z 559.2 to 440.1 for atorvastatin
In our calibration laboratory, which is accredited* by DAkkS according to DIN EN ISO/IEC 17025, we manufacture certified reference materials based on the regulatory issued by NIST (National Institute of Standards and Technology), ASTM (American Society for Testing and Materials) and Pharmacopeias (Ph. Eur., USP). As a result, our certified UV
The HPLC effluent is pumped through a nebulizing needle which is at ground potential. The spray goes through a semi-cylindrical electrode which is at a high potential. The potential difference between the needle and the electrode produces a strong electrical field. This field charges the surface of the liquid and forms a spray of charged droplets.
May 07, 2020 · 3 CN in H 2 O to 95% of CH 3 CN (with 0.1% HCO 2 H) over 6 minutes, followed by 4 minutes isocratic elution, with detection at 495 or 600 nm. Semipreparative RP‐HPLC was performed on an Aijiren system equipped with a Zorbax Eclipse XDB‐C18 RP column (250 × 9.4 mm, 5 μm) with a flow rate 2.0 mL/min, eluting with a gradient of 5% of CH 3 CN in H 2 O to 95% of CH 3 CN (with 0.1% HCO 2
Autosampler Vials, Caps, and Closures. Thermo Scientific™ 20 mm Headspace Vials, Septum, and Caps. Headspace vials are available in clear or amber glass with a round or flat base, a crimp seal with a beveled or square edge finish, or with a screw-thread finish. Headspace vials, septum, and caps can be purchased in various quantities
Vials. Fisher Scientific offers the vials you need for all of your laboratory requirements and applications. Whether your needs include chromatography, sample storage, cryo-preservation or scintillation, we have options for you.