2. Add the internal standard and 10 mL of 1:1 acetone to hexane. 3. Vortex for 1 minute, let it sit for 2 minutes, vortex for an additional minute. 4. Centrifuge at 4,000 RPM for 5 minutes. 5. Transfer the extract into a 15 mL amber vial with a PTFE-lined cap For GC/MS