2019/12/19 · 1.5 mL of 50 mM MgCl2 (clear cap; optional use) 750 reactions kit contains each: One bottle (7.5 mL) of 2X MasterMix (black cap - amber vial) containing Takyon DNA polymerase, MgCl2 (2.5mM final concentration), SYBR Green®, dNTPs, Inert blue dye