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reaction volume at room temperature for 12 h. Products were identified by HPLC-MS based on the corresponding reference compounds (Section V). N. D. = none detected. (a) To 332 µL M9-N medium (pH 7.4) were added 10 µL hemin (1 mM in DMSO), 10 µL 1a
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High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material.
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S1 SUPPLEMENTARY INFORMATION Development of an Automated Kinetic Profiling System with Online HPLC for Reaction Optimization Melodie Christensen,*a,c Folarin Adedeji,a Shane Grosser,a Kerstin Zawatzky,b Yining Ji,a Jinchu Liu,b Jon A. Jurica,a John R. Naber,a and Jason E. Hein*c
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