Article Snippet: A volume of 350 μL of the TEAA buffer was placed into an autosampler vial (Thermo Scientific, Rockwood, TN) followed by 3.50 μL of 0.6 M sodium cyanoborohydride (Sigma) and 3.50 μL of 4% formaldehyde (J.T.
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Article Snippet: A volume of 350 μL of the TEAA buffer was placed into an autosampler vial (Thermo Scientific, Rockwood, TN) followed by 3.50 μL of 0.6 M sodium cyanoborohydride (Sigma) and 3.50 μL of 4% formaldehyde (J.T.
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2016/9/9 · The vial was placed onto the autosampler, which added 100 μL of the internal standard solution (ISTD, 1 g/L in MTBE) and 1.5 mL of an ethanolic KOH solution (1 mol/L). The vial was placed into an agitator set to 80 °C for 40 min and was shaken at a speed of 500 rpm (revolutions per minute).
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3 Using a dropper pipette, a sufficient volume of the lower organic layer was transferred directly into an autosampler vial for GC analysis. Note that it was not necessary to trans-fer the whole extract. Care was taken to ensure that none of the aqueous phase was