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process, selection and use of syringe filters to extend high-performance liquid chromatography (HPLC) column life, minimise extractables and reduce adsorption of active pharmaceutical ingredients. Procuring proper filtration In HPLC, contamination from thein the
Cosmonice Filter How to use : 1. Fill a syringe with the sample you want to filter. 2. Attach a Cosmonice filter to the syringe. 3. Push the syringe plunger to filter the sample. 4. Analyze the
Hydrophilic PTFE syringe filters have broad chemical compatibility and high pH resistance and are optically clear when wet with water. They are versatile filters for use with aqueous and aggressive organic solvent-based solutions and are especially ideal for HPLC sample preparation. The pure.
• Use eluent filters if necessary • Use a degasser – It might be a good idea to degas in an ultrasonic water bath for 5 min • Seal wash – helps the pump seal to survive • If the pump is out of wash solution it should not start • If the pump starts to leak Thermo
To perform this procedure, the syringe filter is attached to a regulated, pressurized source of test fluid and the flow rate is determined from the volume of fluid processed in a specific time interval. Mass Spectrometry The Acrodisc MS syringe filter is eluted with
Clean and Green Minisart® RC Syringe Filter solutions and other membrane options for sample preparation. Minisart® RC syringe filter devices from Sartorius are an excellent, environmental and eco-friendly alternative to PVDF devices. They are just as suitable, and often even better, for HPLC sample preparation than PVDF.
Acrodisc syringe filters with glass fiber 195 ••• Acrodisc syringe filters with GHP membrane 182 •• • • Acrodisc syringe filters with nylon membrane 186 •• • • Acrodisc syringe filters with PTFE membrane 188 •• • • • Acrodisc syringe filters with PVDF membrane
Typically, the first step in selecting a suitable filter is to choose a membrane that is chemically compatible with the sample (see table below for a full selection of mebranes). For aqueous samples, it is preferable to use a hydrophilic membrane (wwPTFE, PVDF
Note: The individually packed sterile syringe filter can be held in the original package to minimize contamination while attaching the syringe. Step 3: Secure the Syringe Filter Secure the syringe filter using a clockwise motion with luer-lock syringe. DO NOT.
5.1.1 After completion of analysis, clean the HPLC as follows. 5.1.2 Transfer the used glassware for washing & keep aside test tube stands lying near the HPLC system. Clean the surrounding area with tissue paper. 5.1.3 Clean the syringe with repeated rinses of distilled water.
I would like to suggest that you review this short article, "Syringe Filter Selection for HPLC or LC/MS samples" for the answer to your question. http://hplctips.blogspot.com/2014/11/syringe
There are three ways to reduce the hold-up volume in a syringe filter. 1. Use a 13 mm syringe filter designed specifically for small volumes instead of a 25 mm filter. 2. Use a syringe filter that has a mini spike outlet. 3. Run a post-filtration air purge on the syringe
Use with: Bases, Most HPLC solvents, Alcohols, Aromatic Hydrocarbons, THF. Don’t use with: Acids, Aggressive Halogenated hydrocarbons, protein samples (Nylon is a high binder) Sterilization: Syringe filters can be sterilized by autoclave at 125° for 15 minutes.
Captiva Premium syringe filters prior to HPLC analysis. The following syringe filter types were evaluated for filtration recovery of food additives. For samples in aqueous media: † Aijiren Captiva Premium PES syringe filter, 25 mm, 0.2 µm (p/n 5190-5098) and 0.45