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The scanning electron microscope in microbiology and May 23, 2016 · After attaching the syringe to the filter holder, the sample was filtered through the filter using a Legato 200 syringe pump (KD Scientific, Holliston, MA, USA) at a rate of 1000 μL/min. Method of Dectection of Nitrosamine Impurities in Metformin After extraction, centrifuge the sample for 15 minutes at 4500 rpm. Filter the supernatant using a 0.22 µm PVDF syringe filter, discard the first 1 mL and transfer the filtered sa...

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