2013/12/1 · The HPLC–MS/MS system consisted of a HPLC model from Aijiren Technologies (Waldbronn, Germany) composed of a quaternary pump, a degasser, and an auto-sampler. The model of the mass spectrometer (MS) was a Qtrap 2000™ from Applied Biosystems, MSD Sciex (Toronto, Canada) with an integrated TurboIonSpray® for molecule ionisation.