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the 10nM to 20mM range. For quantitative analysis of alde-hydes in blood, working standard solutions containing 100nM, 500nM, 1mM, 5mM and 20mM of aldehydes were prepared daily by dilution of the 50mM solution with dis-tilled water. Gas chromatography
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it was frozed and stored at -18oC until analysis. The procedure of sample preparation for analysis involved cheese grating with a fine mesh. Than 3 g of cheese were weighted to 20 ml vial and 1fl of internal standard solution (trans-2-decanal 0.067 μl/ml) was oC for
2015/8/20 · The supernatant and pellet of each NP and AF were digested overnight in a papain buffer (250 μg/ml papain (Sigma-Aldrich) in 50 mM EDTA and 5 mM L-cysteine) at 60 C. GAG content was quantified by using a 1,9-dimethylmethylene blue assay [ 36 ].
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Tri-decanoyl glycerol, cholesteryl myristate and Tri-nonadecanoyl glycerol (Sigma-Aldrich) were used as standards for free and esterified cholesterol and triglycerids, respectively. For fatty acid analysis, FOLCH-extracts were trans-esterified using boron trifluoride-methanol solution (Sigma) at 80°C for 2 hr followed by extraction with hexane.
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The dried extract was dissolved in 1 ml of CDCl 3; an aliquot (0.6 ml) was used for NMR analysis and the remaining sample was stored at -20 C for GC-MS analysis. Polar fraction The supernatant containing the polar intracellular metabolites was freeze-dried.
The GC column used was a 20 m x 0.25 mm x 0.25 mm Rxi-5ms. GC-MS interface temperature was 300 C and (electron impact) ion source temperature was set at 200 C, with 70 V ionization voltages. The mass spectrometer was set to scan m/z range 50-800
2017/4/7 · GC conditions were as follows: 1 μL injection; inlet, 250 °C splitless, carrier 1 mL min −1 He, oven program 70 °C for 3 min, ramp @ 7 °C min −1 to 325 °C, hold 20 min. The transfer line temperature was 290 °C. The detector was operated in electron ionization
Fenpropathrin is a highly potent pyrethroid insecticide used widely for crop farming, and shows significant toxicity to fish 15,16 (see also Sigma-Aldrich's MSDS data, Index-no: 607-239-00-5, fenpropathrin has an LC 50 for rainbow trout of 0.002 μg L −1, 96.0 h).
We offer innovative lipid-based drug delivery excipients, such as Squalene and Synthetic Cholesterol, with superior characteristics enabling you to simplify and streamline formulation processes. You can also take advantage of our customized lipids manufacturing to create a tailored approach for your API.