phase, filtered through a 0.45 syringe filter and injected into HPLC system. RESULTS Chromatographic conditions All the standards were separated within 50 min and showed good resolution between matrix and analyte peaks. Fig 1 shows the
All solvents were of HPLC grade and were filtered using a cellulose filter (0.45 m) prior to use. Prior to HPLC analysis, the samples were passed through a 0.45 m nylon syringe filter (Alltech Assoc) before being manually injected (20 L) each time.
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samples were filtered (0.45 μm PTFE syringe filter). Analysis was performed with PSS WinGPC UniChrom. Sequential batch extractions The same procedure was applied as the normal batch organosolv extraction. After the filtration step, the residue was washed
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3/(m2*h)) to cope with increased production rate and slurry feed Filtrate clarity shall be kept stable (≤ 1 g/l) Moisture content target: less than 45%. 4.1 Results of trials in laboratory and production scale up. First step taken was the analysis of the current used filter media.
Staufen, Germany), and followed by the addition of 20 mL of distilled water and centrifugation (16,000 g, 30 min). The supernatant was filtered through a Sep-Pak C 18 cartridge (WAT023501, Waters) and a Millipore 0.45-syringe filter (PVDF, Whatman,
0.45 μm polyvinylidene difluoride syringe filter (Whatman GmbH, Dassel, Germany), and the concentration of VST International Journal of Nanomedicine downloaded from https://www.dovepress.com/ by 40.77.167.13 on 13-Apr-2021 For personal use only. 1 / 1
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all by Macherey-Nagel, Düren / Germany. 0.45 µm pore size filters of the above types may be attached in front of the 0.2 µm filters if the latter get clogged when used directly. -Note: Check filters for any contamination with analytes of interest. Significant levels of
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3/(m2*h)) to cope with increased production rate and slurry feed Filtrate clarity shall be kept stable (≤ 1 g/l) Moisture content target: less than 45%. 4.1 Results of trials in laboratory and production scale up. First step taken was the analysis of the current used filter media.