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An aliquot of 1 mL of the supernatant was filtered through a 0.2-µm nylon syringe filter into a 1.5-mL microcentrifuge tube and kept at −80 C until the HPLC-ELSD analysis was
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The extract was passed through a nylon syringe filter (0.45 m m pore size, 13 mm diameter) prior to the HPLC-MS determination. LC-MS analysis Analysis was performed using a P200
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